UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 45 / No. 3 / Published : 2009-07

Order : 1, and page :174 - 179

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Original Article :

Comparison of apoptotic index and the amount of brain\'s pyramidal neuron between newborn mice receiving and not receiving docosahexanoic acid during pregnancy

Author :

  1. Muhammad Maksum *1
  2. Hermanto Tri Joewono *2
  3. Widjiati*3
  1. Department of Obstetric and Gynecology, Faculty of Medicine Airlangga University, Dr. Soetomo Hospital, Surabaya
  2. Department of Obstetric and Gynecology, Faculty of Medicine Airlangga University, Dr. Soetomo Hospital, Surabaya
  3. Department of Embryology, Faculty of Veterinary Medicine Airlangga University Surabaya

Abstract :

The main purpose of this experimental study was to compare the apoptotic index and number of the offspring’s Mus musculus brain pyramidal neurons between Intervention and non-Intervention group to docosahexaenoic acid (DHA) during pregnancy. Experimental study, double blind randomized controlled study. Intervention group giving by 20 mg DHA per oral, once daily, in the morning at the same time during early pregnancy until deliver the fetus. After delivery, for both groups, the offspring sacrificed by decapitation and brain pyramidal cells were prepared by double staining with EMX 1 and TUNEL method to calculate apoptotic index and number brain pyramidal neurons. The study conducted at Veterinary Hospital Airlangga University Surabaya after ethical clearance. Laboratory staining was done by Biomedic SOM Brawijaya University Malang. We found that the apoptotic index (2.15 ± 1.19) in Intervention group compare with (6.77 ± 2.92) in control group, and number of pyramidal neurons (149.69 ± 34.53) in Intervention group compare with (101.50 ± 26.14), in control group. According to statistic test the different were significant with p = 0.000 both of those group. The apoptotic index of the Intervention group was significantly lower than non Intervention group and the number pyramidal neurons of the Intervention group was significantly more than non Intervention group.

Keyword :

Mus musculus, pyramidal neuron, apoptotic index, DHA, pregnancy,


References :

Crawford MA,(2000) Placental delivery of arachidonic and docosahexaenoic acid : implication for the lipid nutrition of preterm infant - : American Journal of Clinical Nutrition

Dutta AK,(2000) Transport mechanisms for long-chain polyunsaturated faaty acids in the human placenta - : American Journal of Clinical Nutrition





Archive Article

Cover Media Content

Volume : 45 / No. : 3 / Pub. : 2009-07
  1. Comparison Of Apoptotic Index And The Amount Of Brain\'s Pyramidal Neuron Between Newborn Mice Receiving And Not Receiving Docosahexanoic Acid During Pregnancy
  2. Nucleotide Sequence And Sequence Comparison Of The Repeat Sequence 529 (r529) Of An Indonesian Toxoplasma Gondii Isolate
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